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4.2 Autophagy and cancer Similar to cGAS-STING signaling, the function of autophagy in cancer development and therapy is complex

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For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent